Comparative analyses of stevioside between fresh leaves and in-vitro derived callus tissue from Stevia rebaudiana Bert. using HPLC
نویسندگان
چکیده
منابع مشابه
Identification of Stevioside Using Tissue Culture-Derived Stevia (Stevia rebaudiana) Leaves
Stevioside is a natural sweetener from Stevia leaf, which is 300 times sweeter than sugar. It helps to reduce blood sugar levels dramatically and thus can be of benefit to diabetic people. Tissue culture is a very potential modern technology that can be used in large-scale disease-free stevia production throughout the year. We successfully produced stevia plant through in vitro culture for iden...
متن کاملRapid in vitro propagation of Stevia rebaudiana Bertoni and evaluation of its stevioside content
An efficient protocol was established for rapid in vitro propagation of Stevia rebaudiana Bertoni, using explants from mature plants. The effect of 1/2MS, MS and B5 culture media, also 27 different combinations of auxins and cytokinins was studied on in vitro growth and development of Stevia rebaudiana Bertoni. The best culture medium for shoot and root formation was 1/2MS, producing 1.30 shoot...
متن کاملMutagenicity and human chromosomal effect of stevioside, a sweetener from Stevia rebaudiana Bertoni.
Leaves of Stevia rebaudiana Bertoni have been popularly used as a sweetener in foods and beverages for diabetics and obese people due to their potent sweetener stevioside. In this report, stevioside and steviol were tested for mutagenicity in Salmonella typhimurium strains TA98 and TA100 and for chromosomal effects on cultured human lymphocytes. Stevioside was not mutagenic at concentrations up...
متن کاملTissue Culture and Production of Volatile Secondary Metabolites from Callus of Stevia rebaudiana at Different Combination of Plant Growth Regulators
Stevia rebaudiana Bert (Asteraceae) is herbaceous plant with sweet leaves, which are the source of sweetener products. The present study focuses on effects of different combination of plant growth regulators on callus induction, callus cell growth and regeneration and volatile constituents from callus of stevia in tissue culture condition. The results of present study showed callus formation p...
متن کاملStevioside from Stevia rebaudiana Bertoni Increases Insulin Sensitivity in 3T3-L1 Adipocytes
Stevioside from Stevia rebaudiana has been reported to exert antihyperglycemic effects in both rat and human subjects. There have been few studies on these effects in vitro. In this paper, radioactive glucose uptake assay was implemented in order to assess improvements in insulin sensitivity in 3T3-L1 cells by elevation of glucose uptake following treatment with stevioside. Oil Red-O staining a...
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ژورنال
عنوان ژورنال: Bangladesh Journal of Scientific and Industrial Research
سال: 2015
ISSN: 2224-7157,0304-9809
DOI: 10.3329/bjsir.v49i4.22621